The CD45 or the Leukocyte Common Antigen (LCA-T200) has three exons that can encode the peptide segments and is considered a membrane sialoglycoprotein. As you can splice the exons differently, you can generate various isoforms. It is regarded as a single-chain glycoprotein and is expressed in approximately half of all peripheral CD4+T-cells, as well as half of peripheral B-cells, CD8+T-cells, and leukemic B-cells, as well. The T-cells that express CD45Ra and CD45RO can define complementary, non-overlapping resting peripheral T-cells. They are naïve or virgin white T-cells in the CD45RA and memory T-cells in the CD45RO.
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This antibody is designed for research use and has a clone called SPM504. The immunogen includes stimulated human leucocytes, and the IG isotype is IgG(24). There is no determined epitope, and the CD45RA antibody has a molecular weight between 205 and 220kDa.
It has been human tested and can be used for Immunohistochemistry applications. The procedure for IHC is to use paraffin-embedded or Formalin-fixed tissues, deparaffinizing the slides as necessary. When using the concentrated format, you should dilute the product with a ratio of 1:200, though dilutions are approximate. There is no antigen retrieval method, though you should incubate for at least 30 minutes at room temperature.
The positive control is the tonsil with cellular localization occurring in the membrane.
Forms
This product comes in 1.0 ml, 0.1 ml and 0.5 ml options in a PBS/1 percent BSA buffer with a pH of 7.6. Likewise, it can also be found in a pre-diluted format as 7.0 ml, ready for use in your IHC applications. You shouldn’t let it come in contact with reagents and should never swallow this antibody.
The CD45RA antibody is an excellent way to check various T-cells. Visit Spring Bioscience now for more information.
